Further Purification and Some Properties of Kynureninase.
نویسنده
چکیده
The enzyme kynureninase converts kynurenine and hydroxykynurenine to alanine and anthranilic acid and to alanine and hydroxyanthranilic acid, respectively14>. Previously, Knox 51, Jakoby et al. 4>, a~d Wiss et al. 6> have described the purification and some properties of kynureninase. According to their reports, this enzyme obtained from bacteria was activated with magnesium ions, while it from pig liver was not observed such effect. In the course of the studies on the relation between the increased excretion of xanthurenic acid and the enzyme activities in vitamin Bs deficient rats 7>, the author tried to further purification of this enzyme with DEAE-cellulose column chromatography. The present paper will be described about the purification procedures and some properties of kynureninase prepared from rat and pig livers.
منابع مشابه
Kynureninase from Neurospora: Purification
As part of a study of kynurenine metabolism in Neurospora crassa, the enzyme, kynureninase, has been investigated. Evidence from studies with mycelial pads suggests that tryptophan gives rise to kynurenine, which may be degraded to anthranilic acid (1). Kynurenine is also oxidized to hydroxykynurenine, which is converted to hydroxyanthranilic acid (2). The similarity of these reactions, i.e. th...
متن کاملThe kynureninase of Pseudomonas fluorescens.
Kotake and Nakayama (1) observed the conversion of kynurenine to anthranilic acid and alanine by a mammalian liver extract. Subsequently the enzyme responsible for this reaction in the mammal was partly purified (2,3) and the enzyme, prepared from vitamin Be-deficient animals, was shown to require pyridoxal phosphate for its maximal activity (2). During an investigation of tryptophan metabolism...
متن کاملZiziphus mauritiana mediated synthesis of copper and nickel nanoparticles for comparative efficacy in biological water purification
The burden of life on the earth is the source of biological contamination in water. Nanotechnology has promising contributions in control of microbial contaminations and medicinal plants further increase these properties. Presently, copper acetate and nickel oxide nanoparticles were synthesized using 1mM solution of each with Ziziphus mauritiana leaves extract as reducing agent. Nanoparticles w...
متن کاملIsolation and characterization of low-kynureninase mutants of Neurospora crassa.
Two kynureninase activities are known in Neurospora crassa, one of which (kynureninase I) is inducible, the other (kynureninase II) being constitutive. A method is described for the isolation of low-kynureninase mutants of N. crassa. When grown on an inducer, the mutants show significantly less kynureninase I activity compared with wild type, whereas constitutive kynureninase II activity is una...
متن کاملA Simplified and Reproducible Two-Step Method for the Purification of Prostate-Specific Antigen
Prostate-specific antigen (PSA) was purified to homogeneity from human seminal plasma by ion-exchange chromatography on a CM-Sephadex C-50 and by gel filtration on a Sephacryl S-200 column. A single 33-kDa protein band appeared in SDS-PAGE. High pressure liquid chromatography (HPLC) of the purified protein produced a single peak, while isoelectric focusing demonstrated the presence of five diff...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Nagoya journal of medical science
دوره 26 شماره
صفحات -
تاریخ انتشار 1964